Article
Molecular basis of the ribulose-1,5-bisphosphate carboxylase/oxygenase activase mutation in Arabidopsis thaliana is a guanine-to-adenine transition at the 5'-splice junction of intron 3.
Plant physiology - 1 May 1993
Orozco B M, McClung C R, Werneke J M, Ogren W L
Abstract excerpt
Analysis of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase gene and gene products from Arabidopsis thaliana wild-type plants and the Rubisco activase-deficient mutant strain showed that the rca mutation caused GT to be changed to AT at the 5'-splice junction of intron 3 in the six-intron pre-mRNA. Northern blot analysis, genomic and cDNA sequencing, and primer extension analysis indicated...
Topics
- Amino Acid Sequence
- Arabidopsis
- Base Sequence
- DNA, Complementary
- Genes, Plant
- Introns
- Molecular Sequence Data
- Phenotype
- Plant Proteins
- Point Mutation
- RNA Splicing