Article
Identification of amino acid residues involved in the activity of phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase. A bifunctional enzyme in the alginate biosynthetic pathway of Pseudomonas aeruginosa.
The Journal of biological chemistry - 18 Feb 1994
May T B, Shinabarger D, Boyd A, Chakrabarty A M
Abstract excerpt
Phosphomannose isomerase-guanosine 5'-diphospho-D-mannose pyrophosphorylase (PMI-GMP), which is encoded by the algA gene, catalyzes two noncontiguous steps in the alginate biosynthetic pathway of Pseudomonas aeruginosa; the isomerization of D-fructose 6-phosphate to D-mannose 6-phosphate and the...
Topics
- Alginates
- Amino Acid Sequence
- Bacterial Proteins
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Kinetics
- Mannose-6-Phosphate Isomerase
- Models, Biological
- Molecular Sequence Data
- Multienzyme Complexes
- Mutagenesis, Site-Directed