Article
Clonality analysis of B-cell lymphoma in fresh-frozen and paraffin-embedded tissues: the effects of variable polymerase chain reaction parameters.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc - 1 May 1994
Chen Y T, Whitney K D, Chen Y
Abstract excerpt
To investigate the sensitivity of polymerase chain reaction (PCR)-based detection of B-cell monoclonality and the effects of several variables, we analyzed 119 cases of B-cell lymphomas with proven IgH gene rearrangements, testing fresh-frozen and formalin-fixed materials in parallel. Using fresh...
Topics
- Base Sequence
- Clone Cells
- DNA, Neoplasm
- Frozen Sections
- Genotype
- Humans
- Immunoglobulin Heavy Chains
- Lymphoma, B-Cell
- Molecular Sequence Data
- Paraffin Embedding
- Polymerase Chain Reaction
- Sensitivity and Specificity