Article
Determination of relative amounts of inositol trisphosphate receptor mRNA isoforms by ratio polymerase chain reaction.
The Journal of biological chemistry - 26 Aug 1994
De Smedt H, Missiaen L, Parys J B, Bootman M D, Mertens L, Van Den Bosch L, Casteels R
Abstract excerpt
The relative expression of different inositol 1,4,5-trisphosphate receptor (InsP3R) mRNA was determined in a selection of murine and rat cell types that are commonly used to study InsP3-mediated Ca2+ signaling. Different mRNA species (encoding the known InsP3R isoforms) were co-amplified using co...
Topics
- Animals
- Base Sequence
- Caffeine
- Calcium
- Calcium Channels
- Cells, Cultured
- Cerebellum
- Dose-Response Relationship, Drug
- Genetic Variation
- Inositol 1,4,5-Trisphosphate
- Inositol 1,4,5-Trisphosphate Receptors
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Messenger
- Rats
- Receptors, Cytoplasmic and Nuclear
- Signal Transduction