Article
Involvement of the rml locus in core oligosaccharide and O polysaccharide assembly in Pseudomonas aeruginosa.
Microbiology (Reading, England) - 1 Nov 2000
Rahim Rahim, Burrows Lori L, Monteiro Mario A, Perry Malcolm B, Lam Joseph S
Abstract excerpt
L-Rhamnose (L-Rha) is a component of the lipopolysaccharide (LPS) core, several O antigen polysaccharides, and the cell surface surfactant rhamnolipid of Pseudomonas aeruginosa. In this study, four contiguous genes (rmlBDAC) responsible for the synthesis of dTDP-L-Rha in P. aeruginosa have been cloned and characterized. Non-polar chromosomal rmlC mutants were generated in P. aeruginosa strains PAO1 (serotype O5)...
Topics
- Base Sequence
- Carbohydrate Dehydrogenases
- Carbohydrate Epimerases
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA Primers
- Genes, Bacterial
- Hydro-Lyases
- Mutation
- Nucleotidyltransferases
