Article
Mutagenesis of a gene encoding a cytochrome o-like terminal oxidase of Azotobacter vinelandii: a cytochrome o mutant is aero-tolerant during nitrogen fixation.
FEMS microbiology letters - 15 Jun 1994
Leung D, van der Oost J, Kelly M, Saraste M, Hill S, Poole R K
Abstract excerpt
The amino acid sequence obtained by translating the nucleotide sequence of a 0.55 kb fragment, amplified from Azotobacter vinelandii chromosomal DNA by PCR, was 57% identical to part of the Escherichia coli cyoB gene, encoding subunit I of the cytochrome bo-type quinol oxidase. This fragment was mutated in vitro by insertion of a kanamycin-resistance cassette and introduced into the chromosome of A. vinelandii by...
Topics
- Amino Acid Sequence
- Azotobacter vinelandii
- Base Sequence
- Chlorpromazine
- Cloning, Molecular
- Electron Transport Complex IV
- Molecular Sequence Data
- Mutation
- Nitrogen Fixation
- Oxygen Consumption