Article
Study of cytochrome bo function in Vitreoscilla using a cyo(-) knockout mutant.
Journal of biochemistry - 1 Jul 2000
Kim K J, Chi P Y, Hwang K W, Stark B C, Webster D A
Abstract excerpt
The bacterium, Vitreoscilla, produces a delta mu(Na+) across its membrane during respiration. A key enzyme for this function is the cytochrome bo terminal oxidase which, when incorporated into synthetic proteoliposomes, pumps Na(+) across the membrane upon the addition of a substrate. A Vitreoscilla cytochrome bo knock out (cyo(-)) mutant was isolated by transposon mutagenesis using pUT-mini-Tn5Cm. The membranes...
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