Cloning and mutagenesis of genes encoding the cytochrome bd terminal oxidase complex in Azotobacter vinelandii: mutants deficient in the cytochrome d complex are unable to fix nitrogen in air.

doi:10.1128/jb.172.10.6010-6019.1990

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Article

Cloning and mutagenesis of genes encoding the cytochrome bd terminal oxidase complex in Azotobacter vinelandii: mutants deficient in the cytochrome d complex are unable to fix nitrogen in air.

Journal of bacteriology - 1 Oct 1990

Kelly M J, Poole R K, Yates M G, Kennedy C

Abstract excerpt

The genome of Azotobacter vinelandii contains DNA sequences homologous to the structural genes for the Escherichia coli cytochrome bd terminal oxidase complex. Two recombinant clones bearing cydA- and cydB-like sequence were isolated from an A. vinelandii gene library and subcloned into the plasm...

Topics

Azotobacter
Blotting, Southern
Cloning, Molecular
Cytochrome b Group
Cytochrome d Group
Cytochromes
DNA Transposable Elements
DNA, Bacterial
Dithionite
Electron Transport Chain Complex Proteins
Electron Transport Complex IV
Escherichia coli Proteins
Genes, Bacterial

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Kinetics

Mutation

Nitrogen Fixation

Nucleic Acid Hybridization

Oxidoreductases

Source

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Identifiers

PMID: 2170336
DOI: 10.1128/jb.172.10.6010-6019.1990
PMCID: PMC526923

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2170336-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.