Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase.

doi:10.1021/bi00193a013

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Article

Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase.

Biochemistry - 12 Jul 1994

Brune M, Hunter J L, Corrie J E, Webb M R

Abstract excerpt

A probe has been developed that can rapidly measure micromolar concentrations of inorganic phosphate (Pi), in particular to follow the release of Pi in real time from enzymes such as phosphatases. Its application is described to investigate the mechanism of actomyosin subfragment 1 ATPase. The pr...

Topics

Adenosine Triphosphate
Carrier Proteins
Coumarins
Escherichia coli
Fluorescent Dyes
Kinetics
Mutagenesis, Site-Directed
Mutation
Myosin Subfragments
Myosins
Osmolar Concentration
Phosphate-Binding Proteins
Phosphates
Spectrometry, Fluorescence

Source

Open in PubMed

Identifiers

PMID: 8031761
DOI: 10.1021/bi00193a013

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8031761-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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