Article
The expression of functional link protein in a baculovirus system: analysis of mutants lacking the A, B and B' domains.
The Biochemical journal - 1 Jun 1994
Grover J, Roughley P J
Abstract excerpt
Functional recombinant human link protein has been produced using a baculovirus expression system. In addition to the intact link protein, three mutant forms have also been expressed. Each mutant bears a deletion equivalent to the protein encoded by one exon in the gene. These deletions represent...
Topics
- Aggrecans
- Amidohydrolases
- Amino Acid Sequence
- Animals
- Baculoviridae
- Base Sequence
- Blotting, Western
- Cells, Cultured
- Cloning, Molecular
- DNA, Complementary
- Electrophoresis, Polyacrylamide Gel
- Extracellular Matrix Proteins
- Humans
- Lectins, C-Type
- Molecular Sequence Data
- Moths
- Mutation
- Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase