Article
Simple and rapid detection of cholesteryl ester transfer protein deficiency by using nonradioisotopic single-strand conformation polymorphism analysis.
Clinical chemistry - 1 Dec 1994
Arai T, Tsukada T, Nakayama T
Abstract excerpt
We developed a polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) analysis technique by using silver staining to detect the G-->A point mutation of intron 14 of the cholesteryl ester transfer protein (CETP) gene, which is a common mutation in CETP deficiency. After con...
Topics
- Base Sequence
- Carrier Proteins
- Cholesterol Ester Transfer Proteins
- Cholesterol, HDL
- DNA
- Glycoproteins
- Heterozygote
- Homozygote
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single-Stranded Conformational
- Silver Staining