A new detection method for the K variant of butyrylcholinesterase based on PCR primer introduced restriction analysis (PCR-PIRA).

doi:10.1136/jmg.31.7.576

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Article

A new detection method for the K variant of butyrylcholinesterase based on PCR primer introduced restriction analysis (PCR-PIRA).

Journal of medical genetics - 1 Jul 1994

Shibuta K, Abe M, Suzuki T

Abstract excerpt

The K variant of human butyrylcholinesterase is caused by a G/A transition in the butyrylcholinesterase gene, which neither creates nor destroys any restriction site. In an attempt to detect the K variant both simply and rapidly, we developed a two step method of "PCR primer introduced restrictio...

Topics

Alleles
Asian People
Base Sequence
Butyrylcholinesterase
DNA Probes
Gene Frequency
Humans
Japan
Molecular Sequence Data
Point Mutation
Polymerase Chain Reaction

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