Article
Long-term genome stability and minimal genotypic and phenotypic alterations in HPV16 E7-, but not E6-, immortalized human uroepithelial cells.
Genes & development - 15 Sept 1994
Reznikoff C A, Belair C, Savelieva E, Zhai Y, Pfeifer K, Yeager T, Thompson K J, DeVries S, Bindley C, Newton M A
Abstract excerpt
Parameters of genome instability and morphological alterations associated with cell transformation were studied in an isogeneic set of clonal human uroepithelial cell (HUC) lines immortalized by the human papilloma virus 16 (HPV16) E6 and/or E7 gene(s). HPV16 E6 binds p53, leading to rapid degradation of p53, whereas E7 binds and alters pRb and other proteins. We report that two independent E7-immortalized HUC...
Topics
- Base Sequence
- Cell Line
- Cell Transformation, Viral
- DNA Primers
- DNA, Viral
- Epithelium
- Genotype
- Humans
- In Situ Hybridization, Fluorescence
- Karyotyping
- Models, Biological