Article
The contribution of the cell wall to a transmembrane calcium gradient could play a key role in Bacillus subtilis protein secretion.
Molecular microbiology - 1 Sept 1993
Petit-Glatron M F, Grajcar L, Munz A, Chambert R
Abstract excerpt
A weak Ca(2+)-binding site (Ka = 0.8 x 10(3) M-1, at pH 7) was identified in the mature part of levansucrase. An amino acid substitution (Thr-236-->Ile) in this site alters simultaneously the affinity for calcium, the folding transition and the efficiency of the secretion process of levansucrase. Moreover, the ability of the Bacillus subtilis cell wall to concentrate calcium ions present in the culture medium was...
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