Article
The contribution of the cell wall to a transmembrane calcium gradient could play a key role in Bacillus subtilis protein secretion.
Molecular microbiology - 1 Sept 1993
Petit-Glatron M F, Grajcar L, Munz A, Chambert R
Abstract excerpt
A weak Ca(2+)-binding site (Ka = 0.8 x 10(3) M-1, at pH 7) was identified in the mature part of levansucrase. An amino acid substitution (Thr-236-->Ile) in this site alters simultaneously the affinity for calcium, the folding transition and the efficiency of the secretion process of levansucrase....
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Binding Sites
- Calcium
- Cell Membrane
- Cell Wall
- Genetic Variation
- Hexosyltransferases
- Kinetics
- Molecular Sequence Data
- Parvalbumins
- Protein Folding
- Protein Structure, Secondary
- Sequence Homology, Amino Acid
- Time Factors