Article
The role of the lipoprotein sorting signal (aspartate +2) in pullulanase secretion.
Molecular microbiology - 1 Sept 1993
Poquet I, Kornacker M G, Pugsley A P
Abstract excerpt
The analyses of hybrid proteins and of deletion and insertion mutations reveal that the only amino acid at the amino-proximal end of the cell surface lipoprotein pullulanase that is specifically required for its extracellular secretion is an aspartate at position +2, immediately after the fatty acylated amino-terminal cysteine. To see whether the requirement for this amino acid is related to its proposed role as...
Topics
- Amino Acid Sequence
- Aspartic Acid
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Genetic Variation
- Glycoside Hydrolases
- Lipoproteins
- Molecular Sequence Data
- Mutagenesis