Article
The general protein-export pathway is directly required for extracellular pullulanase secretion in Escherichia coli K12.
Molecular microbiology - 1 Feb 1991
Pugsley A P, Kornacker M G, Poquet I
Abstract excerpt
Pullulanase is an extracellular, cell surface-anchored lipoprotein produced by Gram-negative bacteria belonging to the genus Klebsiella. Its correct localization in recombinant Escherichia coli requires the products of 14 genes that are linked to the enzyme structural gene in the Klebsiella chromosome. In addition, we show here that six sec genes (secA, secB, secD, secE, secF and secY) are all required for...
Topics
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Biological Transport
- Carrier Proteins
- Cloning, Molecular
- DNA, Bacterial
- Endopeptidases
- Escherichia coli
- Escherichia coli Proteins