Article
Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis.
Molecular microbiology - 1 Jun 1994
McNamara P J, Bradley G A, Songer J G
Abstract excerpt
The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node,...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Corynebacterium Infections
- Corynebacterium pseudotuberculosis
- Gene Targeting
- Goat Diseases
- Goats
- Lymph Nodes
- Male
- Molecular Sequence Data