Article
Chloride ion independence of the Bohr effect in a mutant human hemoglobin beta (V1M+H2deleted).
The Journal of biological chemistry - 30 Sept 1994
Fronticelli C, Pechik I, Brinigar W S, Kowalczyk J, Gilliland G L
Abstract excerpt
A mutant human hemoglobin, beta (V1M+H2 delta), has been constructed. Analysis of the oxygen binding curves obtained at pH 8.3, where the Bohr effect is inoperative, indicates that this mutation results in an additional stabilization of the T-state conformation by 0.9 kcal/mol. The crystal struct...
Topics
- Amino Acid Sequence
- Base Sequence
- Chlorides
- Cloning, Molecular
- Crystallography, X-Ray
- DNA Primers
- Hemoglobins
- Hemoglobins, Abnormal
- Humans
- Molecular Sequence Data
- Mutation
- Oxygen
- Protein Binding
- Protein Conformation