Article
Properties of carbohydrate-free recombinant glycogenin expressed in an Escherichia coli mutant lacking UDP-glucose pyrophosphorylase activity.
FEBS letters - 26 Sept 1994
Alonso M D, Lomako J, Lomako W M, Whelan W J, Preiss J
Abstract excerpt
Glycogenin, the self-glucosylating primer for glycogen synthesis, is expressed in wild-type E. coli as a recombinant protein in an already partly glucosylated form, owing to the presence of its substrate, UDP-glucose. By using an E. coli mutant strain lacking in UDP-glucose pyrophosphorylase acti...
Topics
- Escherichia coli
- Gene Expression
- Glucose
- Glucosyltransferases
- Glycogen
- Glycoproteins
- Glycosylation
- Mutation
- Recombinant Proteins
- UTP-Glucose-1-Phosphate Uridylyltransferase
- Uridine Diphosphate Glucose
- Uridine Diphosphate Xylose