Article
Capillary electrophoresis as a tool for optimization of multiplex PCR reactions.
Fresenius' journal of analytical chemistry - 1 Feb 2001
Butler J M, Ruitberg C M, Vallone P M
Abstract excerpt
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling conditions. The process of optimizing a multiplex PCR reaction...
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