Article
Isolation and sequence determination of porcine class II DRB alleles amplified by PCR.
Animal genetics - 1 Apr 1994
Våge D I, Olsaker I, Lingaas F, Lie O
Abstract excerpt
The first domain exon of a porcine DRB gene was amplified by the polymerase chain reaction (PCR), and the nucleotide sequence was determined. In a material consisting of 10 unrelated animals, five different alleles were identified, all probably belonging to a single locus designated DRB1. In addi...
Topics
- Alleles
- Animals
- Base Sequence
- DNA
- HLA-DR Antigens
- HLA-DRB1 Chains
- Histocompatibility Antigens Class II
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Pseudogenes
- Sequence Analysis, DNA