Article
Systematic analysis of swine leukocyte antigen-DRB1 nucleotide polymorphisms using genomic DNA-based high-resolution genotyping and identification of new alleles.
Tissue antigens - 1 Jun 2011
Thong L Minh, Choi H, Kwon O-J, Kim J-H, Kim Y-B, Oh J-W, Seo K, Yeom S-C, Lee W-J, Park C
Abstract excerpt
In an attempt to enable comprehensive high-resolution genotyping of the swine leukocyte antigen (SLA) gene, we performed a systemic analysis of nucleotide polymorphisms at introns 1 and 2 and exon 2 from diverse alleles of SLA-DRB1 and DRB1 pseudogenes. We amplified and cloned 16 partial sequences of SLA-DRB1 and DRB2 introns 1 and 2 from different alleles, and analyzed them together with sequences of four...
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