Article
Potential roles for the glnB and ntrYX genes in Azospirillum brasilense.
FEMS microbiology letters - 24 Jul 2001
Vitorino J C, Steffens M B, Machado H B, Yates M G, Souza E M, Pedrosa F O
Abstract excerpt
Three Azospirillum brasilense mutants constitutive for nitrogen fixation (Nif(C)) in the presence of NH4(+) and deficient in nitrate-dependent growth were used as tools to define the roles of the glnB and ntrYX genes in this organism. Mutant HM14 was complemented for nitrate-dependent growth and NH4(+) regulation of nitrogenase by plasmid pL46 which contains the ntrYX genes of A. brasilense. Mutant HM26 was...
Topics
- Ammonia
- Azospirillum brasilense
- Cloning, Molecular
- Conjugation, Genetic
- Enzyme Activation
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes
- Genes, Bacterial
- Genetic Complementation Test
- Glutamate-Ammonia Ligase
- Mutation
- Nitrates
- Nitrogen Fixation
- Nitrogenase
- Phenotype
- Plasmids
- beta-Galactosidase