Article
The surface exclusion system of RP1: investigation of the roles of trbJ and trbK in the surface exclusion, transfer, and slow-growth phenotypes.
Plasmid - 1 Nov 1994
Lyras D, Chan A W, McFarlane J, Stanisich V A
Abstract excerpt
The contiguous trbJ and trbK genes of RP1 were cloned individually to study their effects. Surface exclusion was conferred only by trbK and only when gene dosage was high or when trbJ was also present in cis or in trans. This suggests that in the low-copy-number RP1, surface exclusion is due to a two-gene interaction in which trbK is the dominant partner. Among surface exclusion genes, trbJ is novel in yielding a...
Topics
- Bacterial Proteins
- Cell Division
- Chromosome Mapping
- Cloning, Molecular
- Conjugation, Genetic
- DNA, Bacterial
- Escherichia coli
- Gene Amplification
- Genes, Bacterial
- Genetic Vectors
- Phenotype