Article
Kinetic analysis of the non-phosphorylated, in vitro phosphorylated, and phosphorylation-site-mutant (Asp8) forms of intact recombinant C4 phosphoenolpyruvate carboxylase from sorghum.
European journal of biochemistry - 15 Feb 1995
Duff S M, Andreo C S, Pacquit V, Lepiniec L, Sarath G, Condon S A, Vidal J, Gadal P, Chollet R
Abstract excerpt
Steady-state kinetic analyses were performed on the non-phosphorylated, in vitro phosphorylated and phosphorylation-site mutant (Ser8-->Asp) forms of purified recombinant sorghum C4 phosphoenolpyruvate (P-pyruvate) carboxylase (EC 4.1.1.31) containing an intact N-terminus. Significant differences in certain kinetic parameters were observed between these three enzyme forms when activity was assayed at a suboptimal...
Topics
- Edible Grain
- Hydrogen-Ion Concentration
- Kinetics
- Mutation
- Phosphoenolpyruvate Carboxykinase (GTP)
- Phosphorylation
- Recombinant Proteins