Article
Positive selection for resistance to 2-deoxyglucose gives rise, in Streptococcus salivarius, to seven classes of pleiotropic mutants, including ptsH and ptsI missense mutants.
Molecular microbiology - 1 Sept 1994
Gauthier L, Thomas S, Gagnon G, Frenette M, Trahan L, Vadeboncoeur C
Abstract excerpt
We have used the toxic non-metabolizable glucose/mannose analogue 2-deoxyglucose to isolate a comprehensive collection of mutants of the phosphoenolpyruvate:sugar phosphotransferase system from Streptococcus salivarius. To increase the range of possible mutations, we isolated spontaneous mutants...
Topics
- Bacterial Proteins
- Base Sequence
- Deoxyglucose
- Drug Resistance, Microbial
- Fructose
- Galactose
- Immunoelectrophoresis
- Lactose
- Molecular Sequence Data
- Mutation
- Phosphoenolpyruvate Sugar Phosphotransferase System
- Phosphotransferases (Nitrogenous Group Acceptor)
- Selection, Genetic
- Streptococcus