Coisogenic all-plus-one immunization: a model for identifying missing proteins in null-mutant conditions. Antibodies to dystrophin in mdx mouse after transplantation of muscle from normal coisogenic donor.

Abstract excerpt

Specific antibody response against an alien protein is one of the basic immunologic mechanisms in immunecompetent organisms. They can be used as a first step in various approaches leading to the identification of proteins or even an antigen-encoding gene. Accordingly, we wanted to find out whether a null-mutant immunecompetent organism would produce specific antibodies against the missing gene product. We chose...

Topics

• Animals
• Antibodies
• Antibody Specificity
• Blotting, Western
• Child
• Chromosome Deletion
• Dystrophin
• Humans
• Immune Tolerance
• Male
• Mice