Article
Chemiluminescent detection of blotted PCR products (CB-PCR) of two CAG dynamic mutations (Huntington's disease and spinocerebellar ataxia type 1).
Journal of medical genetics - 1 Aug 1994
Castellví-Bel S, Matilla T, Banchs M I, Kruyer H, Corral J, Milà M, Estivill X
Abstract excerpt
We have used a non-isotopic PCR assay based on the chemiluminescent detection of blotted PCR products (CB-PCR) for two dynamic mutation diseases (Huntington's disease and spinocerebellar ataxia type 1). This gives an accurate sizing of alleles and permits a rapid analysis of at risk persons. The system involves PCR of the samples, separation of alleles on polyacrylamide gels, Southern blotting, and hybridisation...
Topics
- Alleles
- Base Sequence
- Blotting, Southern
- Electrophoresis, Polyacrylamide Gel
- Fluorescein-5-isothiocyanate
- Humans
- Huntington Disease
- Luminescent Measurements
- Minisatellite Repeats
- Molecular Sequence Data