Article
Standardized microtiter assay for determination of syncytium-inducing phenotypes of clinical human immunodeficiency virus type 1 isolates.
Journal of clinical microbiology - 1 Sept 1994
Japour A J, Fiscus S A, Arduino J M, Mayers D L, Reichelderfer P S, Kuritzkes D R
Abstract excerpt
A standardized assay in 96-well microtiter plates for syncytium-inducing (SI) human immunodeficiency virus type 1 phenotype detection using MT-2 cells has been developed. SI variants were found in 67% of the patients with advanced human immunodeficiency virus disease. The occurrence of the SI phenotype increased with lower CD4+ counts. There was no association between p24 antigenemia and the SI phenotype.
Topics
- Blood Preservation
- CD4 Lymphocyte Count
- Cell Fusion
- Cryopreservation
- Cytopathogenic Effect, Viral
- HIV Core Protein p24
- HIV Infections
- HIV-1
- Humans
- Phenotype
- Reference Standards