Syncytium induction in primary CD4+ T-cell lines from normal donors by human immunodeficiency virus type 1 isolates with non-syncytium-inducing genotype and phenotype in MT-2 cells.

Abstract excerpt

Human immunodeficiency virus type 1 (HIV-1) isolates classified as syncytium-inducing (SI) or non-SI (NSI) in the MT-2 T-cell line exhibit characteristic sequence differences in the V1-V2 and V3 regions of the env gene. Seven HIV-1 isolates were phenotyped as NSI or SI in the MT-2 cell line. Unexpectedly, all four NSI viruses induced large syncytia 4 to 8 days postinoculation in a panel of five primary CD4+...

Topics

• Amino Acid Sequence
• CD4-Positive T-Lymphocytes
• Cell Line
• Coculture Techniques
• Consensus Sequence
• Flow Cytometry
• Genotype
• Giant Cells
• HIV Envelope Protein gp120
• HIV Seronegativity