Article
PCR for capsular typing of Haemophilus influenzae.
Journal of clinical microbiology - 1 Oct 1994
Falla T J, Crook D W, Brophy L N, Maskell D, Kroll J S, Moxon E R
Abstract excerpt
A PCR method for the unequivocal assignment of Haemophilus influenzae capsular type (types a to f) was developed. PCR primers were designed from capsule type-specific DNA sequences cloned from the capsular gene cluster of each of the six capsular types. PCR product was amplified only from the capsular type for which the primers were designed. Product was confirmed by using either an internal oligonucleotide or...
Topics
- Bacterial Capsules
- Base Sequence
- DNA, Bacterial
- Genotype
- Haemophilus influenzae
- Molecular Sequence Data
- Polymerase Chain Reaction