Article
[The isolation and phenotypic characteristics of heat-resistant cells from the Chinese hamster CHO-K1 line].
Tsitologiia - 1 Jan 1994
Konstantinova M F, Nisman B Kh, Ignatova T N
Abstract excerpt
By sequential selection for a resistance to a short-term heating at 45 degrees C, two independent clones, CHSR5 and CHSR6, have been isolated from ovarian cells of the Chinese hamster (CHO-K1). A subline CHO-A40, capable of proliferation at 40 degrees C, has been obtained as mass culture by culturing CHO-K1 cells for 2 months at 40 degrees C. The lines obtained have no cross-resistance to the temperature used in...
Topics
- Animals
- CHO Cells
- Cell Separation
- Clone Cells
- Colchicine
- Cricetinae
- Cricetulus
- Dactinomycin
- Drug Resistance
- Female
- Hot Temperature
- Phenotype
