Article
Heat-resistant variants of the Chinese hamster ovary cell: alteration of cellular structure and expression of vimentin.
Journal of cellular physiology - 1 Apr 1992
Lee Y J, Hou Z Z, Curetty L, Armour E P, al-Saadi A, Bernstein J, Corry P M
Abstract excerpt
Three heat-resistant mutant cell lines (78-1, 78-2, 78-3) were previously selected from Chinese hamster ovary cells. In this study, we investigated whether the differences in intrinsic thermal sensitivity result from alteration of stress protein levels or cellular structural changes. Although there was no significant difference in the levels of stress proteins, i.e., constitutive HSP70 in wild type and three...
Topics
- Animals
- Blotting, Northern
- CHO Cells
- Cells, Cultured
- Cricetinae
- DNA
- Electrophoresis, Polyacrylamide Gel
- Female
- Fluorescent Antibody Technique
- Gene Expression
- Genetic Variation