Article
Tumor necrosis factor-alpha and interleukin-6 production induced by variations of DR4 polymorphism during the primary mixed lymphocyte reaction.
Transplantation - 27 Dec 1994
Toungouz M, Denys C H, Andrien M E, de Groote D, Dupont E C
Abstract excerpt
Serologically defined MHC class II differences provoke release of TNF-alpha and IL-6 during MLR. In order to assess the influence of micropolymorphism defined at the genomic level, we selected informative donors pairs within DR2 DR4 serologically defined unrelated subjects by combining those differing only by DR4 alleles, as assessed by PCR-SSOP (DRB1*0401 to 07). Two groups of MLR combinations were tested...
Topics
- Alleles
- HLA-DP Antigens
- HLA-DQ Antigens
- HLA-DR4 Antigen
- Humans
- Interleukin-6
- Lipopolysaccharides
- Lymphocyte Culture Test, Mixed
- Lymphocytes
- Polymerase Chain Reaction
- Polymorphism, Genetic