Article
The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli.
Genetics - 1 Apr 1995
Cao Y, Kogoma T
Abstract excerpt
The mechanism of recA polA lethality in Escherichia coli has been studied. Complementation tests have indicated that both the 5'-->3' exonuclease and the polymerization activities of DNA polymerase I are essential for viability in the absence of RecA protein, whereas the viability and DNA replication of DNA polymerase I-defective cells depend on the recombinase activity of RecA. An alkaline sucrose gradient...
Topics
- Bacterial Proteins
- DNA Nucleotidyltransferases
- DNA Polymerase I
- DNA Repair
- DNA Replication
- Escherichia coli
- Genes, Lethal
- Integrases
- Mutation
- Protein Biosynthesis
- Rec A Recombinases