Article
Mutations in the glycerol kinase gene restore the ability of a ptsGHI mutant of Bacillus subtilis to grow on glycerol.
Microbiology (Reading, England) - 1 May 1995
Wehtje Christina, Beijer Lena, Nilsson Rune-Pär, Rutberg Blanka
Abstract excerpt
Although glycerol is not taken up via the phosphotransferase system (PTS) in Bacillus subtilis, some mutations that affect the general components of the PTS impair the ability of cells to grow on glycerol. Five revertants of a pts deletion mutant that grow on glycerol were analysed. They were shown to carry mutations in the glycerol kinase gene. These are missense mutations located in parts of the glpK gene that...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Binding Sites
- DNA Primers
- Escherichia coli
- Gene Deletion
- Genes, Bacterial
- Genes, Suppressor
- Genotype
- Glycerol