Mammalian Sperm DNA Susceptibility to In Situ Denaturation Associated with the Presence of DNA Strand Breaks as Measured by the Terminal Deoxynucleotidyl Transferase Assay

Abstract excerpt

Sperm from four mammalian species were analyzed by the sperm chromatin structure assay (SCSA) and the terminal deoxynucleotidyl transferase assay (TdTA) using flow cytometry. The SCSA quantitates the susceptibility of sperm nuclear DNA to in situ acid denaturation, while the TdTA quantitates the presence of endogenous DNA strand breaks in sperm nuclear chromatin. Correlations were seen between the percentage of...

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