Article
Sperm chromatin structure assay (SCSA®).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Evenson Donald P
Abstract excerpt
The SCSA(®) is the pioneering assay for the detection of damaged sperm DNA and altered proteins in sperm nuclei via flow cytometry of acridine orange (AO) stained sperm. The SCSA(®) is considered to be the most precise and repeatable test providing very unique, dual parameter data (red vs. green fluorescence) on a 1,024 × 1,024 channel scale, not only on DNA fragmentation but also on abnormal sperm characterized...
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