Article
Expression in Escherichia coli of a gene encoding a thermostable chitinase from Streptomyces thermoviolaceus OPC-520.
Bioscience, biotechnology, and biochemistry - 1 Jan 1995
Tsujibo H, Endo H, Miyamoto K, Inamori Y
Abstract excerpt
An expression plasmid for a thermostable chitinase gene from S. thermoviolaceus OPC-520 in E. coli was constructed. A cloned chitinase (Chi40) was purified from the periplasmic space of E. coli harboring the expression plasmid. The N-terminal sequence of Chi40 was 11 amino acids longer than that...
Topics
- Amino Acid Sequence
- Asparagine
- Catalysis
- Chitinases
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Enzyme Stability
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Glutamine
- Hot Temperature
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Reference Standards
- Streptomyces