Article
Toxicity studies on Reactive Blue-2 leached from affinity material exposed to extreme chemical conditions.
Bioseparation - 1 Oct 1994
Bertrand O, Boschetti E, Cochet S, Girot P, Hebert E, Monsigny M, Roche A C, Santambien P, Sdiqui N
Abstract excerpt
Toxicity effects related to leached ligands from affinity sorbents that can contaminate biological preparations were investigated in the particular case of immobilized Reactive Blue-2. Initially, identification of the real chemical structure of leached dye has been done by HPLC after incubation in extreme conditions. Toxicity investigations in vitro involving several well known tests showed no toxic effects...
Topics
- Cell Adhesion
- Cell Line
- Cell Survival
- Chromatography, High Pressure Liquid
- Coloring Agents
- Escherichia coli
- Flow Cytometry
- HeLa Cells
- Humans
- Hydrolysis
- Ligands