Article
cry1 genes from Bacillus thuringiensis: specificity determination and implications for primer design.
Biotechnology letters - 1 Dec 2009
Davolos Camila C, Guidelli-Thuler Ana M, de Abreu Irlan L, Sena Janete A D, Lemos Manoel V F
Abstract excerpt
Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic sequences from five cry1 subclasses was carried out and characterized by molecular tools. The...
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