Article
Genome-wide screening reveals the genetic determinants of an antibiotic insecticide in Bacillus thuringiensis.
The Journal of biological chemistry - 10 Dec 2010
Liu Xiao-Yan, Ruan Li-Fang, Hu Zhen-Fei, Peng Dong-Hai, Cao Shi-Yun, Yu Zi-Niu, Liu Yao, Zheng Jin-Shui, Sun Ming
Abstract excerpt
Thuringiensin is a thermostable secondary metabolite in Bacillus thuringiensis and has insecticidal activity against a wide range of insects. Until now, the regulatory mechanisms and genetic determinants involved in thuringiensin production have remained unclear. Here, we successfully used heterologous expression-guided screening in an Escherichia coli-Bacillus thuringiensis shuttle bacterial artificial...
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