Article
Real-time PCR for rapid genotyping of angiotensin-converting enzyme insertion/deletion polymorphism.
Clinical biochemistry - 1 Nov 2001
Lin M H, Tseng C H, Tseng C C, Huang C H, Chong C K, Tseng C P
Abstract excerpt
OBJECTIVE: To develop a real-time PCR technique for detection of the insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) gene. DESIGN AND METHODS: Three primers were designed for performing real-time PCR in the presence of SYBR Green I as flurochrome followed by melting curve analysis. Forty human genomic DNA that have been genotyped by two-rounds of conventional PCR were used for...
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