Article
Identification of two major HLA-B44 subtypes and a novel B44 sequence (B*4404). Oligotyping and solid phase sequencing of polymerase chain reaction products.
Human immunology - 1 Jan 1995
Yao Z, Keller E, Scholz S, McNicholas A, Volgger A, Albert E D
Abstract excerpt
PCR-based analyses were performed for the identification of HLA-B44 subgroups. Genomic DNA from six homozygous cell lines and 44 healthy individuals who had serologically tested positive for HLA-B44 was investigated for polymorphism in exons 2 and 3 of the HLA-B44 genes. Two primers were designed...
Topics
- Alleles
- Base Sequence
- Cell Line
- Consensus Sequence
- Exons
- Feasibility Studies
- HLA-B Antigens
- HLA-B44 Antigen
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Nucleic Acid