Article
Analysis of HLA-B*44 alleles encoded on extended HLA haplotypes by direct automated sequencing.
Tissue antigens - 1 Oct 1994
Petersdorf E W, Setoda T, Smith A G, Hansen J A
Abstract excerpt
We developed a PCR-based approach to sequence exons 2 and 3 of HLA-B44 alleles from genomic DNA. We applied this method to determine the B44 alleles encoded on extended HLA-A, B, DRB1, DQB1 haplotypes and the degree of mismatching for B44 alleles among marrow transplant patients and their unrelated donors (URD). A total of 81 samples was studied and included 38 patients, 42 donors and the cell "FMB"; the 80...
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