Article
Analysis of HLA-B*44 alleles encoded on extended HLA haplotypes by direct automated sequencing.
Tissue antigens - 1 Oct 1994
Petersdorf E W, Setoda T, Smith A G, Hansen J A
Abstract excerpt
We developed a PCR-based approach to sequence exons 2 and 3 of HLA-B44 alleles from genomic DNA. We applied this method to determine the B44 alleles encoded on extended HLA-A, B, DRB1, DQB1 haplotypes and the degree of mismatching for B44 alleles among marrow transplant patients and their unrelat...
Topics
- Alleles
- Amino Acid Sequence
- Consensus Sequence
- Genes, MHC Class I
- HLA-B Antigens
- HLA-B44 Antigen
- Haplotypes
- Histocompatibility Testing
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid