Article
A panel of monoclonal antibodies to rat plectin: distinction by epitope mapping and immunoreactivity with different tissues and cell lines.
Acta histochemica - 1 Dec 1994
Foisner R, Feldman B, Sander L, Seifert G, Artlieb U, Wiche G
Abstract excerpt
A panel of twelve monoclonal antibodies (mAbs) to plectin was analyzed to localize molecular epitopes and assess their utility for various immunotechniques. Based on staining patterns obtained by immunoblotting of proteolytic plectin fragments five groups of mAbs with spatially closely linked epitopes were distinguished. In combination with previous results obtained with recombinant mutant proteins, the epitopes...
Topics
- Animals
- Antibodies, Monoclonal
- Cattle
- Cell Line
- Cricetinae
- Enzyme-Linked Immunosorbent Assay
- Epitope Mapping
- Fibroblasts
- Fluorescent Antibody Technique
- Glioma
- Humans
