Article
Chick laminin: isolation by monoclonal antibodies and differential distribution of variants in the embryo.
Experimental cell research - 1 Dec 1991
Brubacher D, Wehrle-Haller B, Chiquet M
Abstract excerpt
In order to study the expression and function of laminin variants during chick embryonic development, we have generated monoclonal antibodies against chick heart laminin. One monoclonal antibody (mAb), called 9/F-10, could be used to purify chick laminin to homogeneity. By rotary shadowing, cross-shaped and T-shaped laminin particles as well as aggregates of two laminin molecules crosslinked via their short arms...
Topics
- Animals
- Antibodies
- Antibodies, Monoclonal
- Chick Embryo
- Chickens
- Chromatography, Affinity
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Immunoblotting
- Immunohistochemistry
- Laminin
