Article
Cryptic dehalogenase and chloroamidase genes in Pseudomonas putida and the influence of environmental conditions on their expression.
Archives of microbiology - 1 Jan 1995
Hope S J, Slater J H
Abstract excerpt
Mutants of two strains of Pseudomonas putida expressed two cryptic chloroamidases (C-amidase and H-amidase) and one cryptic dehalogenase (DehII). The mutants were selected on either 2-chloropropionamide (2CPA) or 2-monochloropropionate (2MCPA), developing as papillae in parental colonies growing on a metabolisable support substrate. Mutants expressing C-amidase were selected if 2CPA was utilised as either a...
Topics
- Acetamides
- Amides
- Amidohydrolases
- Cell Division
- Culture Media
- Gene Expression
- Genes, Bacterial
- Hydrocarbons, Chlorinated
- Hydrogen-Ion Concentration
- Hydrolases
- Mutation