Article
The hinge deletion allelic variant of porcine IgA results from a mutation at the splice acceptor site in the first C alpha intron.
Journal of immunology (Baltimore, Md. : 1950) - 15 Apr 1995
Brown W R, Kacskovics I, Amendt B A, Blackmore N B, Rothschild M, Shinde R, Butler J E
Abstract excerpt
Recently published genomic and cDNA sequences for porcine IgA suggested that the splice acceptor site in the C alpha 1-C alpha 2 intron was an AA rather than an AG dinucleotide. This possibility was tested in an in vitro HeLa cell splicing system using an RNA substrate corresponding to the genomic DNA with the putative AA splice site. Data indicated that splicing occurred at a cryptic AG site 12 nucleotides into...
Topics
- Alleles
- Animals
- Base Sequence
- Female
- Genes, Immunoglobulin
- Immunoglobulin A
- Immunoglobulin alpha-Chains
- Male
- Molecular Sequence Data
- Polymorphism, Restriction Fragment Length