Article
Mutant genes of gramicidin S synthetase 1 defective in phenylalanine racemization have the same sequence as the wild gene.
Journal of biochemistry - 1 Dec 1994
Hori K, Saito F, Tokita K, Kurotsu T, Kanda M, Saito Y
Abstract excerpt
Mutant grs1 genes were cloned and sequenced from the Bacillus brevis Nagano BI-4, C-3, E-1, and E-2 strains, which produce defective gramicidin S synthetase 1 (GS1), lacking racemase activity. Surprisingly, these mutant genes had entirely the same sequence as that of the wild type gene. These mutant strains also produce defective gramicidin S synthetase 2 (GS2), lacking 4'-phosphopantetheine, a prosthetic group...
Topics
- Amino Acid Isomerases
- Amino Acid Sequence
- Bacillus
- Bacterial Proteins
- Cloning, Molecular
- Molecular Sequence Data
- Multienzyme Complexes
- Mutation
- Peptide Synthases
- Phenylalanine
- Racemases and Epimerases